Previously unidentified changes in renal cell carcinoma gene expression identified by parametric analysis of microarray data.

ABSTRACT:

Background

Renal cell carcinoma is a common malignancy that often presents as a metastatic-disease for which there are no effective treatments. To gain insights into the mechanism of renal cell carcinogenesis, a number of genome-wide expression profiling studies have been performed. Surprisingly, there is very poor agreement among these studies as to which genes are differentially regulated. To better understand this lack of agreement we profiled renal cell tumor gene expression using genome-wide microarrays (45,000 probe sets) and compare our analysis to previous microarray studies.

Methods

We hybridized total RNA isolated from renal cell tumors and adjacent normal tissue to Affymetrix U133A and U133B arrays. We removed samples with technical defects and removed probesets t

SUBMITTER: Lenburg ME 

PROVIDER: S-EPMC317310 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature