Biochemical characterization of trans-sialidase TS1 variants from Trypanosoma congolense.

ABSTRACT:

Background

Animal African trypanosomiasis, sleeping sickness in humans and Nagana in cattle, is a resurgent disease in Africa caused by Trypanosoma parasites. Trans-sialidases expressed by trypanosomes play an important role in the infection cycle of insects and mammals. Whereas trans-sialidases of other trypanosomes like the American T. cruzi are well investigated, relatively little research has been done on these enzymes of T. congolense.

Results

Based on a partial sequence and an open reading frame in the WTSI database, DNA sequences encoding for eleven T. congolense trans-sialidase 1 variants with 96.3% overall amino acid identity were amplified. Trans-sialidase 1 variants were expressed as recombinant proteins, isolated and assayed for trans-sialylation activity. The pu

SUBMITTER: Koliwer-Brandl H 

PROVIDER: S-EPMC3173295 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature