Identification of histamine receptors and effects of histamine on murine and simian colonic excitability.

ABSTRACT:

Background

Inflammatory responses can include recruitment of cells of hematopoietic origin to the tunica muscularis. These cells can secrete a variety of factors which can reset the gain of smooth muscle cells (SMC) and influence motor patterns. Histamine (H), a major mediator in inflammation, is released by mast cells and exerts diverse effects in SMC by binding to H receptors. The profiles of H receptor expression in animal models used to study inflammatory diseases are unknown.

Methods

Histamine receptor expression and electro-mechanical responses to H were tested in simian and murine colonic smooth muscle using qualitative and quantitative PCR, isometric force measurements, microelectrode recordings and patch clamp techniques.

Key results

H1, H2, and H4 receptor t

SUBMITTER: Kim H 

PROVIDER: S-EPMC3173574 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature