Human amniotic fluid as a potential new source of organ specific precursor cells for future regenerative medicine applications.

ABSTRACT:

Purpose

Human amniotic fluid contains multiple cell types, including pluripotent and committed progenitor cells, and fully differentiated cells. We characterized various cell populations in amniotic fluid.

Materials and methods

Optimum culture techniques for multiple cell line passages with minimal morphological change were established. Cell line analysis and characterization were done with reverse transcriptase and real-time polymerase chain reaction. Immunoseparation was done to distinguish native progenitor cell lines and their various subpopulations.

Results

Endodermal and mesodermal marker expression was greatest in samples of early gestational age while ectodermal markers showed a constant rate across all samples. Pluripotent and mesenchymal cells were always pr

SUBMITTER: Da Sacco S 

PROVIDER: S-EPMC3174101 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature