Molecular mining of alleles in water buffalo Bubalus bubalis and characterization of the TSPY1 and COL6A1 genes.

ABSTRACT:

Background

Minisatellites are an integral part of eukaryotic genomes and show variation in the complexity of their organization. Besides their presence in non-coding regions, a small fraction of them are part of the transcriptome, possibly participating in gene regulation, expression and silencing. We studied the minisatellite (TGG)(n) tagged transcriptome in the water buffalo Bubalus bubalis across various tissues and the spermatozoa, and characterized the genes TSPY1 and COL6A1 discovered in the process.

Results

Minisatellite associated sequence amplification (MASA) conducted using cDNA and oligonucleotide primer (TGG)(5) uncovered 38 different mRNA transcripts from somatic tissues and gonads and 15 from spermatozoa. These mRNA transcripts corresponded to several known and

SUBMITTER: Kumar S 

PROVIDER: S-EPMC3174239 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature