Reiterative Recombination for the in vivo assembly of libraries of multigene pathways.

ABSTRACT: The increasing sophistication of synthetic biology is creating a demand for robust, broadly accessible methodology for constructing multigene pathways inside of the cell. Due to the difficulty of rationally designing pathways that function as desired in vivo, there is a further need to assemble libraries of pathways in parallel, in order to facilitate the combinatorial optimization of performance. While some in vitro DNA assembly methods can theoretically make libraries of pathways, these techniques are resource intensive and inherently require additional techniques to move the DNA back into cells. All previously reported in vivo assembly techniques have been low yielding, generating only tens to hundreds of constructs at a time. Here, we develop "Reiterative Recombination," a robust metho

SUBMITTER: Wingler LM 

PROVIDER: S-EPMC3174608 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature