Experimental annotation of post-translational features and translated coding regions in the pathogen Salmonella Typhimurium.

ABSTRACT:

Background

Complete and accurate genome annotation is crucial for comprehensive and systematic studies of biological systems. However, determining protein-coding genes for most new genomes is almost completely performed by inference using computational predictions with significant documented error rates (> 15%). Furthermore, gene prediction programs provide no information on biologically important post-translational processing events critical for protein function.

Results

We experimentally annotated the bacterial pathogen Salmonella Typhimurium 14028, using "shotgun" proteomics to accurately uncover the translational landscape and post-translational features. The data provide protein-level experimental validation for approximately half of the predicted protein-coding genes i

SUBMITTER: Ansong C 

PROVIDER: S-EPMC3174948 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature