Distinct populations of hepatic stellate cells in the mouse liver have different capacities for retinoid and lipid storage.

ABSTRACT:

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Hepatic stellate cell (HSC) lipid droplets are specialized organelles for the storage of retinoid, accounting for 50-60% of all retinoid present in the body. When HSCs activate, retinyl ester levels progressively decrease and the lipid droplets are lost. The objective of this study was to determine if the HSC population in a healthy, uninjured liver demonstrates heterogeneity in its capacity for retinoid and lipid storage in lipid droplets. To this end, we utilized two methods of HSC isolation, which leverage distinct properties of these cells, including their vitamin A content and collagen expression. HSCs were isolated either from wild type (WT) mice in the C57BL/6 genetic background by flotation in a Nycodenz density gradient, followed by fluorescence activated cell s

SUBMITTER: D'Ambrosio DN 

PROVIDER: S-EPMC3174979 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature