Diagnosis of lung cancer in individuals with solitary pulmonary nodules by plasma microRNA biomarkers.

ABSTRACT:

Background

Making a definitive preoperative diagnosis of solitary pulmonary nodules (SPNs) found by CT has been a clinical challenge. We previously demonstrated that microRNAs (miRNAs) could be used as biomarkers for lung cancer diagnosis. Here we investigate whether plasma microRNAs are useful in identifying lung cancer among individuals with CT-detected SPNs.

Methods

By using quantitative reverse transcriptase PCR analysis, we first determine plasma expressions of five miRNAs in a training set of 32 patients with malignant SPNs, 33 subjects with benign SPNs, and 29 healthy smokers to define a panel of miRNAs that has high diagnostic efficiency for lung cancer. We then validate the miRNA panel in a testing set of 76 patients with malignant SPNs and 80 patients with benign S

SUBMITTER: Shen J 

PROVIDER: S-EPMC3175224 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature