EFS shows biallelic methylation in uveal melanoma with poor prognosis as well as tissue-specific methylation.

ABSTRACT:

Background

Uveal melanoma (UM) is a rare eye tumor. There are two classes of UM, which can be discriminated by the chromosome 3 status or global mRNA expression profile. Metastatic progression is predominantly originated from class II tumors or from tumors showing loss of an entire chromosome 3 (monosomy 3). We performed detailed EFS (embryonal Fyn-associated substrate) methylation analyses in UM, cultured uveal melanocytes and normal tissues, to explore the role of the differentially methylated EFS promoter region CpG island in tumor classification and metastatic progression.

Methods

EFS methylation was determined by direct sequencing of PCR products from bisulfite-treated DNA or by sequence analysis of individual cloned PCR products. The results were associated with clinic

SUBMITTER: Neumann LC 

PROVIDER: S-EPMC3175225 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature