Hemolytic phospholipase C inhibition protects lung function during Pseudomonas aeruginosa infection.

ABSTRACT:

Rationale

The opportunistic pathogen Pseudomonas aeruginosa causes both acute and chronic lung infections and is particularly problematic in patients with cystic fibrosis and those undergoing mechanical ventilation. Decreased lung function contributes significantly to morbidity and mortality during P. aeruginosa infection, and damage inflicted by P. aeruginosa virulence factors contributes to lung function decline.

Objectives

We sought to describe direct contribution of a bacterial phospholipase C/sphingomyelinase, PlcHR, to alteration of host lung physiology and characterize a potential therapeutic for protection of lung function.

Methods

We infected C57Bl/6 mice with P. aeruginosa wild-type or isogenic plcHR deletion strains and measured lung function using computer

SUBMITTER: Wargo MJ 

PROVIDER: S-EPMC3175536 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature