Fas determines differential fates of resident and recruited macrophages during resolution of acute lung injury.

ABSTRACT:

Rationale

During acute lung injury (ALI) the macrophage pool expands markedly as inflammatory monocytes migrate from the circulation to the airspaces. As inflammation resolves, macrophage numbers return to preinjury levels and normal tissue structure and function are restored.

Objectives

To determine the fate of resident and recruited macrophages during the resolution of ALI in mice and to elucidate the mechanisms responsible for macrophage removal.

Methods

ALI was induced in mice using influenza A (H1N1; PR8) infection and LPS instillation. Dye labeling techniques, bone marrow transplantation, and surface immunophenotyping were used to distinguish resident and recruited macrophages during inflammation and to study the role of Fas in determining macrophage fate during

SUBMITTER: Janssen WJ 

PROVIDER: S-EPMC3175550 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature