Microarray characterization of human embryonic stem cell--derived retinal cultures.

ABSTRACT:

Purpose

A number of protocols have been published to induce retinal determination from human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC). Although all these studies have shown some degree of expression of markers of retinal cells, fewer than 30 markers are typically used to characterize the ESC-derived retinal cells. Hence, it is not known whether they express all the genes present in normal developing retinal cells. To assess the efficiency of their retinal determination protocol at the transcriptome level and to understand the changes in human retinal gene expression patterns during development, the authors conducted a microarray-based analysis comparing human retina to hESC-derived retinal cells.

Methods

The authors extracted total RNA from 60-da

SUBMITTER: Lamba DA 

PROVIDER: S-EPMC3175935 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature