Sequence variation in the herpes simplex virus U(S)1 ocular virulence determinant.

ABSTRACT:

Purpose

The herpes simplex virus type 1 (HSV-1) U(S)1 gene encodes host-range and ocular virulence determinants. Mutations in U(S)1 affecting virulence are known in strain OD4, but the genomic variation across several strains is not known. The goal was to determine the degree of sequence variation in the gene from several ocular HSV isolates.

Methods

The U(S)1 gene from six ocular HSV-1 isolates, as well as strains KOS and F, were sequenced, and bioinformatics analyses were applied to the data.

Results

Strains 17, F, CJ394, and CJ311 had identical amino acid sequences. With the other strains, most of the variability was concentrated in the amino-terminal third of the protein. MEME analysis identified a 63-residue core sequence (motif 1) present in all α-herpesvirus U(

SUBMITTER: Kolb AW 

PROVIDER: S-EPMC3175936 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature