Characterization of Langerin-expressing dendritic cell subsets in the normal cornea.

ABSTRACT:

Purpose

In addition to Langerhans cells (LCs), other dendritic cells (CD11c(+)) have recently been shown to express Langerin (c-type lectin). In skin, (non-LC) Langerin+ dendritic cells initiate adaptive immunity. However, whether such dendritic cells (DC) reside in the cornea, an immune-privileged tissue, is unknown.

Methods

Normal C57BL/6 corneas were harvested for qRT-PCR analyses of Langerin expression in the epithelium versus stroma. Immunohistochemistry for Langerin was also performed. Single-cell preparations of epithelium versus stroma were FACS analyzed for CD11c, CD11b, and CD103 expression. Fluorescence microscopy of corneas from muLangerin-eGFP mice (in which all CD11c(+) Langerin+ cells express eGFP), huLangerin-DTA mice (only LCs are constitutively deleted), an

SUBMITTER: Hattori T 

PROVIDER: S-EPMC3175952 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature