Very late antigen-1 mediates corneal lymphangiogenesis.

ABSTRACT:

Purpose

To investigate the specific role of very late antigen-1 (VLA-1; also known as integrin α1β1) in corneal inflammatory lymphangiogenesis in vivo and lymphatic endothelial cell functions in vitro.

Methods

A standard suture-induced corneal inflammatory lymphangiogenesis model was used in normal adult BALB/c mice to test the effect of systemic administration of VLA-1-neutralizing antibody on lymphatic formation and macrophage infiltration in vivo. Additionally, a human lymphatic endothelial cell culture system was used to examine the effect of VLA-1 gene depletion on lymphatic endothelial cell functions in vitro using small interfering RNAs.

Results

These data demonstrated, for the first time, that VLA-1 blockade significantly suppressed corneal lymphangiogenesis a

SUBMITTER: Grimaldo S 

PROVIDER: S-EPMC3175962 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature