Hyperoxia therapy of pre-proliferative ischemic retinopathy in a mouse model.

ABSTRACT:

Purpose

To investigate the therapeutic use and mechanisms of action of normobaric hyperoxia to promote revascularization and to prevent neovascularization in a mouse model of oxygen-induced ischemic retinopathy.

Methods

Hyperoxia treatment (HT, 40%-75% oxygen) was initiated on postnatal day (P) 14 during the pre-proliferative phase of ischemic retinopathy. Immunohistochemistry, ELISA, and quantitative PCR were used to assess effects on retinal vascular repair and pathologic angiogenesis in relation to glial cell injury, VEGF protein, and mRNA levels of key mediators of pathologic angiogenesis. Effects of intravitreal injections of VEGF and the VEGF inhibitor VEGFR1/Fc fusion protein were also studied.

Results

Administration of HT during the ischemic pre-proliferative

SUBMITTER: Zhang W 

PROVIDER: S-EPMC3175995 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature