Mouse conjunctival forniceal gene expression during postnatal development and its regulation by Kruppel-like factor 4.

ABSTRACT:

Purpose

To identify the changes in postnatal mouse conjunctival forniceal gene expression and their regulation by Klf4 during the eye-opening stage when the goblet cells first appear.

Methods

Laser microdissection (LMD) was used to collect conjunctival forniceal cells from postnatal (PN) day 9, PN14 and PN20 wild-type (WT), and PN14 Klf4-conditional null (Klf4CN) mice, in which goblet cells are absent, developing, present, and missing, respectively. Microarrays were used to compare gene expression among these groups. Expression of selected genes was validated by quantitative RT-PCR, and spatiotemporal expression was assessed by in situ hybridization.

Results

This study identified 668, 251, 1160, and 139 transcripts that were increased and 492, 377, 1419, and 57 transc

SUBMITTER: Gupta D 

PROVIDER: S-EPMC3176043 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature