Image defocus and altered retinal gene expression in chick: clues to the pathogenesis of ametropia.

ABSTRACT:

Purpose

Because of the retina's role in refractive development, this study was conducted to analyze the retinal transcriptome in chicks wearing a spectacle lens, a well-established means of inducing refractive errors, to identify gene expression alterations and to develop novel mechanistic hypotheses about refractive development.

Methods

One-week-old white Leghorn chicks wore a unilateral spectacle lens of +15 or -15 D for 6 hours or 3 days. With total RNA from the retina/(retinal pigment epithelium, RPE), chicken gene microarrays were used to compare gene expression levels between lens-wearing and contralateral control eyes (n = 6 chicks for each condition). Normalized microarray signal intensities were evaluated by analysis of variance, using a false discovery rate of <10%

SUBMITTER: Stone RA 

PROVIDER: S-EPMC3176046 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature