Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly.

ABSTRACT:

Background

The Australian sheep blowfly Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae) is a destructive pest of the sheep, a model organism for insecticide resistance research, and a valuable tool for medical and forensic professionals. However, genomic information on L. cuprina is still sparse.

Results

We report here the construction of an embryonic and 2 larval cDNA libraries for L. cuprina. A total of 29,816 expressed sequence tags (ESTs) were obtained and assembled into 7,464 unique clusters. The sequence collection captures a great diversity of genes, including those related to insecticide resistance (e.g., 12 cytochrome P450s, 2 glutathione S transferases, and 6 esterases). Compared to Drosophila melanogaster, codon preference is different in 13 of the 18 amino a

SUBMITTER: Lee SF 

PROVIDER: S-EPMC3176259 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature