Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.

ABSTRACT: Nanotechnology is expected to play a vital role in the rapidly developing field of nanomedicine, creating innovative solutions and therapies for currently untreatable diseases, and providing new tools for various biomedical applications, such as drug delivery and gene therapy. In order to optimize the efficacy of nanoparticle (NP) delivery to cells, it is necessary to understand the mechanisms by which NPs are internalized by cells, as this will likely determine their ultimate sub-cellular fate and localisation. Here we have used pharmacological inhibitors of some of the major endocytic pathways to investigate nanoparticle uptake mechanisms in a range of representative human cell lines, including HeLa (cervical cancer), A549 (lung carcinoma) and 1321N1 (brain astrocytoma). Chlorpromazine a

SUBMITTER: dos Santos T 

PROVIDER: S-EPMC3176276 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature