Second-generation high-throughput forward genetic screen in mice to isolate subtle behavioral mutants.

ABSTRACT: Forward genetic screens have been highly successful in revealing roles of genes and pathways in complex biological events. Traditionally these screens have focused on isolating mutants with the greatest phenotypic deviance, with the hopes of discovering genes that are central to the biological event being investigated. Behavioral screens in mice typically use simple activity-based assays as endophenotypes for more complex emotional states of the animal. They generally set the selection threshold for a putative mutant at 3 SDs (z score of 3) from the average behavior of normal animals to minimize false-positive results. Behavioral screens using a high threshold for detection have generally had limited success, with high false-positive rates and subtle phenotypic differences that have made m

SUBMITTER: Kumar V 

PROVIDER: S-EPMC3176609 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature