Determinants of successful CD8+ T-cell adoptive immunotherapy for large established tumors in mice.

ABSTRACT:

Purpose

Adoptive cell transfer (ACT) of tumor infiltrating or genetically engineered T cells can cause durable responses in patients with metastatic cancer. Multiple clinically modifiable parameters can comprise this therapy, including cell dose and phenotype, in vivo antigen restimulation, and common gamma-chain (γ(c)) cytokine support. However, the relative contributions of each these individual components to the magnitude of the antitumor response have yet to be quantified.

Experimental design

To systematically and quantitatively appraise each of these variables, we employed the Pmel-1 mouse model treating large, established B16 melanoma tumors. In addition to cell dose and magnitude of in vivo antigen restimulation, we also evaluated the relative efficacy of central memo

SUBMITTER: Klebanoff CA 

PROVIDER: S-EPMC3176721 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature