Discovery of DNA viruses in wild-caught mosquitoes using small RNA high throughput sequencing.

ABSTRACT:

Background

Mosquito-borne infectious diseases pose a severe threat to public health in many areas of the world. Current methods for pathogen detection and surveillance are usually dependent on prior knowledge of the etiologic agents involved. Hence, efficient approaches are required for screening wild mosquito populations to detect known and unknown pathogens.

Methodology/principal findings

In this study, we explored the use of Next Generation Sequencing to identify viral agents in wild-caught mosquitoes. We extracted total RNA from different mosquito species from South China. Small 18-30 bp length RNA molecules were purified, reverse-transcribed into cDNA and sequenced using Illumina GAIIx instrumentation. Bioinformatic analyses to identify putative viral agents were conduc

SUBMITTER: Ma M 

PROVIDER: S-EPMC3176773 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature