Comprehensive insights into Mycobacterium tuberculosis DevR (DosR) regulon activation switch.

ABSTRACT: DevR regulon function is believed to be crucial for the survival of Mycobacterium tuberculosis during dormancy. In this study, we undertook a comprehensive analysis of the DevR regulon. All the regulon promoters were assigned to four classes based on the number of DevR binding sites (Dev boxes). A minimum of two boxes are essential for complete interaction and their tandem arrangement is an architectural hallmark at all promoters. Initial interaction of DevR with the conserved box is essential for its cooperative binding to adjacent sites bearing low to very poor sequence conservation and is the universal mechanism underlying DevR-mediated transcriptional induction. The functional importance of tandem arrangement was established by analyzing promoter variants harboring Dev boxes with alter

SUBMITTER: Chauhan S 

PROVIDER: S-EPMC3177182 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature