Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes.

ABSTRACT: Dhh1 and Pat1 in yeast are mRNA decapping activators/translational repressors thought to play key roles in the transition of mRNAs from translation to degradation. However, little is known about the physical and functional relationships between these proteins and the translation machinery. We describe a previously unknown type of diauxic shift-dependent modulation of the intracellular locations of Dhh1 and Pat1. Like the formation of P bodies, this phenomenon changes the spatial relationship between components involved in translation and mRNA degradation. We report significant spatial separation of Dhh1 and Pat1 from ribosomes in exponentially growing cells. Moreover, biochemical analyses reveal that these proteins are excluded from polysomal complexes in exponentially growing cells, indic

SUBMITTER: Drummond SP 

PROVIDER: S-EPMC3177209 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature