3D-Image analysis platform monitoring relocation of pluripotency genes during reprogramming.

ABSTRACT: Nuclear organization of chromatin is an important level of genome regulation with positional changes of genes occurring during reprogramming. Inherent variability of biological specimens, wide variety of sample preparation and imaging conditions, though pose significant challenges to data analysis and comparison. Here, we describe the development of a computational image analysis toolbox overcoming biological variability hurdles by a novel single cell randomizing normalization. We performed a comparative analysis of the relationship between spatial positioning of pluripotency genes with their genomic activity and determined the degree of similarity between fibroblasts, induced pluripotent stem cells and embryonic stem cells. Our analysis revealed a preferred positioning of actively transcr

SUBMITTER: Jost KL 

PROVIDER: S-EPMC3177216 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature