Control of Nanoscale Environment to Improve Stability of Immobilized Proteins on Diamond Surfaces.

ABSTRACT: Immunoassays for detection of bacterial pathogens rely on the selectivity and stability of bio-recognition elements such as antibodies tethered to sensor surfaces. The search for novel surfaces that improve the stability of biomolecules and assay performance has been pursued for a long time. However, the anticipated improvements in stability have not been realized in practice under physiological conditions because the surface functionalization layers on commonly used substrates, silica and gold, are themselves unstable on time scales of days. In this paper, we show that covalent linking of antibodies to diamond surfaces leads to substantial improvements in biological activity of proteins as measured by the ability to selectively capture cells of the pathogenic bacterium Escherichia coli O1

SUBMITTER: Radadia AD 

PROVIDER: S-EPMC3177702 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature