Sequencing of bovine herpesvirus 4 v.test strain reveals important genome features.

ABSTRACT:

Background

Bovine herpesvirus 4 (BoHV-4) is a useful model for the human pathogenic gammaherpesviruses Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus. Although genome manipulations of this virus have been greatly facilitated by the cloning of the BoHV-4 V.test strain as a Bacterial Artificial Chromosome (BAC), the lack of a complete genome sequence for this strain limits its experimental use.

Methods

In this study, we have determined the complete sequence of BoHV-4 V.test strain by a pyrosequencing approach.

Results

The long unique coding region (LUR) consists of 108,241 bp encoding at least 79 open reading frames and is flanked by several polyrepetitive DNA units (prDNA). As previously suggested, we showed that the prDNA unit located at the left prDNA

SUBMITTER: Palmeira L 

PROVIDER: S-EPMC3178527 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature