Metabolic adaptation in transplastomic plants massively accumulating recombinant proteins.

ABSTRACT:

Background

Recombinant chloroplasts are endowed with an astonishing capacity to accumulate foreign proteins. However, knowledge about the impact on resident proteins of such high levels of recombinant protein accumulation is lacking.

Methodology/principal findings

Here we used proteomics to characterize tobacco (Nicotiana tabacum) plastid transformants massively accumulating a p-hydroxyphenyl pyruvate dioxygenase (HPPD) or a green fluorescent protein (GFP). While under the conditions used no obvious modifications in plant phenotype could be observed, these proteins accumulated to even higher levels than ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), the most abundant protein on the planet. This accumulation occurred at the expense of a limited number of leaf prot

SUBMITTER: Bally J 

PROVIDER: S-EPMC3178635 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature