Deletion of alternative pathways for reductant recycling in Thermococcus kodakarensis increases hydrogen production.

ABSTRACT: Hydrogen (H₂) production by Thermococcus kodakarensis compares very favourably with the levels reported for the most productive algal, fungal and bacterial systems. T. kodakarensis can also consume H₂ and is predicted to use several alternative pathways to recycle reduced cofactors, some of which may compete with H₂ production for reductant disposal. To explore the reductant flux and possible competition for H₂ production in vivo, T. kodakarensis TS517 was mutated to precisely delete each of the alternative pathways of reductant disposal, H₂ production and consumption. The results obtained establish that H₂ is generated predominantly by the membrane-bound hydrogenase complex (Mbh), confirm the essential role of the SurR (TK1086p) regulator in vivo, delineate the roles of sulfur (S°) regulo

SUBMITTER: Santangelo TJ 

PROVIDER: S-EPMC3179246 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature