Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

ABSTRACT:

Background

16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the outcome of microbial community analyses. The aim of this study is to compare differences in output, ease, and cost among three different amplicon pyrosequencing methods for the Roche/454 Titanium platform

Methodology/principal findings

The following three pyrosequencing methods for 16S rRNA genes were selected in this study: Method-1 (standard method) is the recommended method for bi-directional sequencing using the LIB-A kit; Method-2 is a new option designed in this study for unidir

SUBMITTER: Tamaki H 

PROVIDER: S-EPMC3179495 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature