Identification of H-2d restricted T cell epitope of foot-and-mouth disease virus structural protein VP1.

ABSTRACT:

Background

Foot-and-mouth disease (FMD) is a highly contagious and devastating disease affecting livestock that causes significant financial losses. Therefore, safer and more effective vaccines are required against Foot-and-mouth disease virus(FMDV). The purpose of this study is to screen and identify an H-2d restricted T cell epitope from the virus structural protein VP1, which is present with FMD. We therefore provide a method and basis for studying a specific FMDV T cell epitope.

Results

A codon-optimized expression method was adopted for effective expression of VP1 protein in colon bacillus. We used foot-and-mouth disease standard positive serum was used for Western blot detection of its immunogenicity. The VP1 protein was used for immunizing BALB/c mice, and spleen lymp

SUBMITTER: Liu XS 

PROVIDER: S-EPMC3179754 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature