Genome-wide expression assay comparison across frozen and fixed postmortem brain tissue samples.

ABSTRACT:

Background

Gene expression assays have been shown to yield high quality genome-wide data from partially degraded RNA samples. However, these methods have not yet been applied to postmortem human brain tissue, despite their potential to overcome poor RNA quality and other technical limitations inherent in many assays. We compared cDNA-mediated annealing, selection, and ligation (DASL)- and in vitro transcription (IVT)-based genome-wide expression profiling assays on RNA samples from artificially degraded reference pools, frozen brain tissue, and formalin-fixed brain tissue.

Results

The DASL-based platform produced expression results of greater reliability than the IVT-based platform in artificially degraded reference brain RNA and RNA from frozen tissue-based samples. Althoug

SUBMITTER: Chow ML 

PROVIDER: S-EPMC3179967 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature