Smooth muscle calponin: an unconventional CArG-dependent gene that antagonizes neointimal formation.

ABSTRACT:

Objective

Smooth muscle calponin (CNN1) contains multiple conserved intronic CArG elements that bind serum response factor and display enhancer activity in vitro. The objectives here were to evaluate these CArG elements for activity in transgenic mice and determine the effect of human CNN1 on injury-induced vascular remodeling.

Methods and results

Mice carrying a lacZ reporter under control of intronic CArG elements in the human CNN1 gene failed to show smooth muscle cell (SMC)-restricted activity. However, deletion of the orthologous sequences in mice abolished endogenous Cnn1 promoter activity, suggesting their necessity for in vivo Cnn1 expression. Mice carrying a 38-kb bacterial artificial chromosome (BAC) harboring the human CNN1 gene displayed SMC- restricted expressio

SUBMITTER: Long X 

PROVIDER: S-EPMC3179981 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature