Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I.

ABSTRACT:

Background

The spontaneous metamorphosis of the polychaete Capitella sp. I larvae into juveniles requires minor morphological changes, including segment formation, body elongation, and loss of cilia. In this study, we investigated changes in the expression patterns of both proteins and phosphoproteins during the transition from larvae to juveniles in this species. We used two-dimensional gel electrophoresis (2-DE) followed by multiplex fluorescent staining and MALDI-TOF mass spectrometry analysis to identify the differentially expressed proteins as well as the protein and phosphoprotein profiles of both competent larvae and juveniles.

Results

Twenty-three differentially expressed proteins were identified in the two developmental stages. Expression patterns of two of those pr

SUBMITTER: Chandramouli KH 

PROVIDER: S-EPMC3180302 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature