Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis.

ABSTRACT:

Background

Yersinia pestis is the causative agent of plague. The two transcriptional regulators, PhoP and RovA, are required for the virulence of Y. pestis through the regulation of various virulence-associated loci. They are the global regulators controlling two distinct large complexes of cellular pathways.

Methodology/principal findings

Based on the LacZ fusion, primer extension, gel mobility shift, and DNase I footprinting assays, RovA is shown to recognize both of the two promoters of its gene in Y. pestis. The autoregulation of RovA appears to be a conserved mechanism shared by Y. pestis and its closely related progenitor, Y. pseudotuberculosis. In Y. pestis, the PhoP regulator responds to low magnesium signals and then negatively controls only one of the two promoters

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3180457 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature