Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut.

ABSTRACT:

Background

Wild peanut species (Arachis spp.) are a rich source of new alleles for peanut improvement. Plant transcriptome analysis under specific experimental conditions helps the understanding of cellular processes related, for instance, to development, stress response, and crop yield. The validation of these studies has been generally accomplished by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) which requires normalization of mRNA levels among samples. This can be achieved by comparing the expression ratio between a gene of interest and a reference gene which is constitutively expressed. Nowadays there is a lack of appropriate reference genes for both wild and cultivated Arachis. The identification of such genes would allow a consistent analysis of qRT-

SUBMITTER: Morgante CV 

PROVIDER: S-EPMC3180468 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature