Refining transcriptional programs in kidney development by integration of deep RNA-sequencing and array-based spatial profiling.

ABSTRACT:

Background

The developing mouse kidney is currently the best-characterized model of organogenesis at a transcriptional level. Detailed spatial maps have been generated for gene expression profiling combined with systematic in situ screening. These studies, however, fall short of capturing the transcriptional complexity arising from each locus due to the limited scope of microarray-based technology, which is largely based on "gene-centric" models.

Results

To address this, the polyadenylated RNA and microRNA transcriptomes of the 15.5 dpc mouse kidney were profiled using strand-specific RNA-sequencing (RNA-Seq) to a depth sufficient to complement spatial maps from pre-existing microarray datasets. The transcriptional complexity of RNAs arising from mouse RefSeq loci was catalo

SUBMITTER: Thiagarajan RD 

PROVIDER: S-EPMC3180702 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature