Visualization of mRNA localization in Xenopus oocytes.

ABSTRACT: Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution.

SUBMITTER: Gagnon JA 

PROVIDER: S-EPMC3181151 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature