Deterministic and stochastic population-level simulations of an artificial lac operon genetic network.

ABSTRACT:

Background

The lac operon genetic switch is considered as a paradigm of genetic regulation. This system has a positive feedback loop due to the LacY permease boosting its own production by the facilitated transport of inducer into the cell and the subsequent de-repression of the lac operon genes. Previously, we have investigated the effect of stochasticity in an artificial lac operon network at the single cell level by comparing corresponding deterministic and stochastic kinetic models.

Results

This work focuses on the dynamics of cell populations by incorporating the above kinetic scheme into two Monte Carlo (MC) simulation frameworks. The first MC framework assumes stochastic reaction occurrence, accounts for stochastic DNA duplication, division and partitioning and tracks

SUBMITTER: Stamatakis M 

PROVIDER: S-EPMC3181209 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature