Quantitative analysis of the effect of cancer invasiveness and collagen concentration on 3D matrix remodeling.

ABSTRACT: Extracellular matrix (ECM) remodeling is a key component of cell migration and tumor metastasis, and has been associated with cancer progression. Despite the importance of matrix remodeling, systematic and quantitative studies on the process have largely been lacking. Furthermore, it remains unclear if the disrupted tensional homeostasis characteristic of malignancy is due to initially altered ECM and tissue properties, or to the alteration of the tissue by tumor cells. To explore these questions, we studied matrix remodeling by two different prostate cancer cell lines in a three-dimensional collagen system. Over one week, we monitored structural changes in gels of varying collagen content using confocal reflection microscopy and quantitative image analysis, tracking metrics of fibril frac

SUBMITTER: Harjanto D 

PROVIDER: S-EPMC3181246 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature