Radiation modulation of microRNA in prostate cancer cell lines.

ABSTRACT:

Background

MicroRNAs (miRNA) are gene regulators and play an important role in response to cellular stress.

Methods

Using multiplexed quantitative real-time PCR we performed global miRNA screening of prostate cancer cells in response to radiation treatment.

Results

Several miRNA were significantly altered in response to radiation treatment. Significant changes were observed in miR-521 and miR-34c. To determine the role of miR-521 in radiation response we transiently overexpressed miR-521 using miR-521 mimic. The miR-521 mimic significantly sensitized prostate cancer cells to radiation treatment. Conversely, ectopic inhibition of miR-521 resulted in radiation resistance of prostate cancer cells. To determine the mechanism by which miR-521 modulates radiation sensitivit

SUBMITTER: Josson S 

PROVIDER: S-EPMC3182144 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature