Video-rate bioluminescence imaging of matrix metalloproteinase-2 secreted from a migrating cell.

ABSTRACT:

Background

Matrix metalloproteinase-2 (MMP-2) plays an important role in cancer progression and metastasis. MMP-2 is secreted as a pro-enzyme, which is activated by the membrane-bound proteins, and the polarized distribution of secretory and the membrane-associated MMP-2 has been investigated. However, the real-time visualizations of both MMP-2 secretion from the front edge of a migration cell and its distribution on the cell surface have not been reported.

Methodology/principal findings

The method of video-rate bioluminescence imaging was applied to visualize exocytosis of MMP-2 from a living cell using Gaussia luciferase (GLase) as a reporter. The luminescence signals of GLase were detected by a high speed electron-multiplying charge-coupled device camera (EM-CCD camera) w

SUBMITTER: Suzuki T 

PROVIDER: S-EPMC3182179 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature