High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism.

ABSTRACT: The pathways that comprise cellular metabolism are highly interconnected, and alterations in individual enzymes can have far-reaching effects. As a result, global profiling methods that measure gene expression are of limited value in predicting how the loss of an individual function will affect the cell. In this work, we employed a new method of global phenotypic profiling to directly define the genes required for the growth of Mycobacterium tuberculosis. A combination of high-density mutagenesis and deep-sequencing was used to characterize the composition of complex mutant libraries exposed to different conditions. This allowed the unambiguous identification of the genes that are essential for Mtb to grow in vitro, and proved to be a significant improvement over previous approaches. To fu

SUBMITTER: Griffin JE 

PROVIDER: S-EPMC3182942 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature