Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors.

ABSTRACT: Nuclear import of karyophilic proteins is carried out by a variety of mechanisms. We previously showed that two basic helix-loop-helix proteins, NeuroD1 and E47, synergistically affect each other's nuclear import. In this study, we dissected the molecular pathways underlying nuclear import of the NeuroD1/E47 heterodimer. In vitro nuclear import assays indicated that importin α family members are the major nuclear import receptors for E47. However, inhibition of importin α resulted in cytoplasmic retention of E47 that could be rescued by its binding partner, NeuroD1, through heterodimerization. In addition, nuclear import of NeuroD1 was importin α independent but importin β1 dependent. In primary neurons, localization of endogenous E47 was not affected by importin α inhibition, suggesting t

SUBMITTER: Mehmood R 

PROVIDER: S-EPMC3183024 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature