Activation of the mitochondrial permeability transition pore modulates Ca2+ responses to physiological stimuli in adult neurons.

ABSTRACT: The participation of mitochondria in cellular and neuronal Ca(2+) homeostatic networks is now well accepted. Yet, critical tests of specific mitochondrial pathways in neuronal Ca(2+) responses have been hampered because the identity of mitochondrial proteins that must be integrated within this dynamic system remain uncertain. One putative pathway for Ca(2+) efflux from mitochondria exists through the formation of the permeability transition pore (PTP) that is often associated with cellular and neuronal death. Here, we have evaluated neuronal Ca(2+) dynamics and the PTP in single adult neurons in wild-type mice and those missing cyclophilin D (CyPD), a key regulator of the PTP. Using high-resolution time-lapse imaging, we demonstrate that PTP opening only follows simultaneous activation wit

SUBMITTER: Barsukova A 

PROVIDER: S-EPMC3183752 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature