Native ligands change integrin sequestering but not oligomerization in raft-mimicking lipid mixtures.

ABSTRACT: Distinct lipid environments, including lipid rafts, are increasingly recognized as a crucial factor affecting membrane protein function in plasma membranes. Unfortunately, an understanding of their role in membrane protein activation and oligomerization has remained elusive due to the challenge of characterizing these often small and transient plasma membrane heterogeneities in live cells. To address this difficulty, we present an experimental model membrane platform based on polymer-supported lipid bilayers containing stable raft-mimicking domains (type I) and homogeneous cholesterol-lipid mixtures (type II) into which transmembrane proteins are incorporated (α(v)β(3) and α(5)β(1) integrins). These flexible lipid platforms enable the use of confocal fluorescence spectroscopy, including th

SUBMITTER: Siegel AP 

PROVIDER: S-EPMC3183796 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature