PITX2 is involved in stress response in cultured human trabecular meshwork cells through regulation of SLC13A3.

ABSTRACT:

Purpose

Mutations of the PITX2 gene cause Axenfeld-Rieger syndrome (ARS) and glaucoma. In this study, the authors investigated genes directly regulated by the PITX2 transcription factor to gain insight into the mechanisms underlying these disorders.

Methods

RNA from nonpigmented ciliary epithelium cells transfected with hormone-inducible PITX2 and activated by mifepristone was subjected to microarray analyses. Data were analyzed using dCHIP algorithms to detect significant differences in expression. Genes with significantly altered expression in multiple microarray experiments in the presence of activated PITX2 were subjected to in silico and biochemical analyses to validate them as direct regulatory targets. One target gene was further characterized by studying the effect o

SUBMITTER: Strungaru MH 

PROVIDER: S-EPMC3183983 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature