A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum.

ABSTRACT:

Background

The halophilic archaeon Halobacterium salinarum expresses bacteriorhodopsin, a retinal-protein that allows photosynthetic growth. Transcription of the bop (bacterioopsin) gene is controlled by two transcription factors, Bat and Brz that induce bop when cells are grown anaerobically and under light.

Results

A new gene was identified that is transcribed together with the brz gene that encodes a small basic protein designated as Brb (bacteriorhodopsin-regulating basic protein). The translation activity of the start codon of the brb gene was confirmed by BgaH reporter assays. In vivo site-directed mutagenesis of the brb gene showed that the Brb protein cooperates with Brz in the regulation of bop expression. Using a GFP reporter assay, it was demonstrated that Brb coo

SUBMITTER: Tarasov V 

PROVIDER: S-EPMC3184054 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature